Coding

Part:BBa_K4491005:Design

Designed by: Kazimir Uzwyshyn Jones   Group: iGEM22_Cambridge   (2022-10-10)


AS16 (anti-sigma factor to ECF16)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 420
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The anti-sigma factor AS16 antagonises transcription factor ECF16. ECF16 otherwise activates transcription at a promoter of ECF16 (p_ECF16)


Source

Rhodus et al., 2013

References

Rhodus et al., 2013